{"id":384,"date":"2017-07-21T16:23:13","date_gmt":"2017-07-21T16:23:13","guid":{"rendered":"https:\/\/www.bic.nus.edu.sg\/lsicorp\/?page_id=384"},"modified":"2023-08-17T08:12:42","modified_gmt":"2023-08-17T08:12:42","slug":"advanced-imaging-laboratory","status":"publish","type":"page","link":"https:\/\/www.nus.edu.sg\/lsi\/advanced-imaging-laboratory\/","title":{"rendered":"Advanced Imaging Laboratory (AIL)"},"content":{"rendered":"<p><strong>About the Advanced Imaging Laboratory<\/strong><\/p>\n<p>The Advanced Imaging Laboratory is a core microscopy facility of the Life Sciences Institute. Light microscopes in the facility are available for fluorescence, live-cell timelapse and confocal imaging. These are complemented with specialised software for processing and analysis of multi-dimensional images.<\/p>\n<h3><\/h3>\n<p><strong>Microscopes:<\/strong><\/p>\n<ul>\n<li>Leica SP5 inverted confocal microscope<\/li>\n<li>Zeiss LSM510 upright confocal microscope<\/li>\n<li>Zeiss AxioImager Z1 upright fluorescence microscope<\/li>\n<li>Olympus IX81 inverted fluorescence microscope with stage-top live-cell imaging chamber<\/li>\n<li>Olympus FV3000RS inverted confocal<\/li>\n<li>Zeiss PALM \u2013 LCM laser microdissection<\/li>\n<\/ul>\n<h3><\/h3>\n<p><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"280\" class=\"size-full wp-image-387 alignleft\" style=\"float: left; margin-right: 15px; max-width: 280px;\" src=\"https:\/\/www.nus.edu.sg\/lsi\/wp-content\/uploads\/2017\/07\/z1-t.jpg\" srcset=\"https:\/\/www.nus.edu.sg\/lsi\/wp-content\/uploads\/2017\/07\/z1-t.jpg 280w, https:\/\/www.nus.edu.sg\/lsi\/wp-content\/uploads\/2017\/07\/z1-t-150x150.jpg 150w\" sizes=\"(max-width: 280px) 100vw, 280px\" \/>Both the confocal microscopes are equipped with the following lasers: 405nm, 488nm, 543nm and 633nm. In addition the LSM510 has 458nm and 514nm laser lines.The two wide-field fluorescence microscopes are equipped with filters for blue, green, red and far red fluorescence.<\/p>\n<p>The laser scanning cytometer uses an inverted microscope to acquire images which are then analysed in a format that is similar to flow cytometry data.<\/p>\n<p>Training is provided for the use of the microscopes and software for processing and analysing microscope images. The software available includes Imaris, MetaMorph and Fiji.<\/p>\n<h3><\/h3>\n<p><strong>Training<\/strong><\/p>\n<p>To arrange a training session on any of the microscopes, please fill up the training request form by clicking <a href=\"http:\/\/tinyurl.com\/IPTRform1\">here<\/a>.<\/p>\n<p>To enquire more about the services at LSI Histology Core, please contact Miss Syaza Hazwany Mohammad Azhar or Dr Lim Hwee Ying at <a href=\"mailto:lsibox10@nus.edu.sg\">lsibox10@nus.edu.sg<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>About the Advanced Imaging Laboratory The Advanced Imaging Laboratory is a core microscopy facility of the Life Sciences Institute. Light microscopes in the facility are available for fluorescence, live-cell timelapse and confocal imaging. These are complemented with specialised software for processing and analysis of multi-dimensional images. Microscopes: Leica SP5 inverted confocal microscope Zeiss LSM510 upright<a class=\"read-more\" href=\"https:\/\/www.nus.edu.sg\/lsi\/advanced-imaging-laboratory\/\">Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"custompagewithmenu.php","meta":{"footnotes":""},"tags":[29],"class_list":["post-384","page","type-page","status-publish","hentry","tag-mm-ailfc"],"_links":{"self":[{"href":"https:\/\/www.nus.edu.sg\/lsi\/wp-json\/wp\/v2\/pages\/384"}],"collection":[{"href":"https:\/\/www.nus.edu.sg\/lsi\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.nus.edu.sg\/lsi\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.nus.edu.sg\/lsi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nus.edu.sg\/lsi\/wp-json\/wp\/v2\/comments?post=384"}],"version-history":[{"count":10,"href":"https:\/\/www.nus.edu.sg\/lsi\/wp-json\/wp\/v2\/pages\/384\/revisions"}],"predecessor-version":[{"id":2265,"href":"https:\/\/www.nus.edu.sg\/lsi\/wp-json\/wp\/v2\/pages\/384\/revisions\/2265"}],"wp:attachment":[{"href":"https:\/\/www.nus.edu.sg\/lsi\/wp-json\/wp\/v2\/media?parent=384"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nus.edu.sg\/lsi\/wp-json\/wp\/v2\/tags?post=384"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}